Brain Tissue Oxygen: In Vivo Monitoring with Carbon Paste Electrodes

نویسندگان

  • Fiachra B. Bolger
  • John P. Lowry
چکیده

In this communication we review selected experiments involving the use of carbon paste electrodes (CPEs) to monitor and measure brain tissue O2 levels in awake freely-moving animals. Simultaneous measurements of rCBF were performed using the H2 clearance technique. Voltammetric techniques used include both differential pulse (O2) and constant potential amperometry (rCBF). Mild hypoxia and hyperoxia produced rapid changes (decrease and increase respectively) in the in vivo O2 signal. Neuronal activation (tail pinch and stimulated grooming) produced similar increases in both O2 and rCBF indicating that CPE O2 currents provide an index of increases in rCBF when such increases exceed O2 utilization. Saline injection produced a transient increase in the O2 signal while chloral hydrate produced slower more long-lasting changes that accompanied the behavioral changes associated with anaesthesia. Acetazolamide increased O2 levels through an increase in rCBF.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Long-Term Monitoring of Brain Dopamine Metabolism In Vivo with Carbon Paste Electrodes

This review focuses on the stability of voltammetric signals recorded over periods of months with carbon paste electrodes (CPEs) implanted in the brain. The key interaction underlying this stability is between the pasting oil and brain lipids that are capable of inhibiting the fouling caused by proteins. In brain regions receiving a significant dopaminergic input, a peak due to the methylated m...

متن کامل

Ion Selective Carbon Paste Electrode For The Determination Of Flavoxate Muscle Relaxant Drug In Pharmaceutical Formulation

Abstract The utility of carbon paste electrode for the determination of flavoxate HCl modified with flavoxate-tetraphenylborate (FLX-TPB) and flavoxate-phosphotungestic acid (FLX-PTA) ion-pairs in batch mode is demonstrated. The electrodes revealed a Nernstian response over a wide concentration ranges 1.39×10-5-1x10-2 mol L-1 and1×10-5-1x10-2 mol L-1 using FLX-TPB and FLX-PTA, respectively. The...

متن کامل

Development of novel potentiometric sensors for determination of lidocaine hydrochloride in pharmaceutical preparations, serum and urine samples

This article is focused on the determination of lidocaine hydrochloride as a local anaesthetic drug. A potentiometric method based on modified screen-printed and modified carbon paste ion-selective electrodes was described for the determination of lidocaine hydrochloride in different pharmaceutical preparations. It was based on potentiometric titration of lidocaine hydrochloride using modified ...

متن کامل

Development of novel potentiometric sensors for determination of lidocaine hydrochloride in pharmaceutical preparations, serum and urine samples

This article is focused on the determination of lidocaine hydrochloride as a local anaesthetic drug. A potentiometric method based on modified screen-printed and modified carbon paste ion-selective electrodes was described for the determination of lidocaine hydrochloride in different pharmaceutical preparations. It was based on potentiometric titration of lidocaine hydrochloride using modified ...

متن کامل

Bienzyme Carbon Paste Electrodes for L-Glutamate Determination

playing an important role in various areas, such as amino acid biosynthesis, bioprocess monitoring, the food industry, and in medicine. It is widely used as a flavor enhancer in various foodstuffs and seasonings (1), as well as being connected to the Chinese restaurant syndrome (2). It has been associated with several diseases; hence, it is used as a clinical marker for general and protein meta...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2005